PoplarV1, Main, Exploration, bibRecord, 001095

A 5-Enolpyruvylshikimate 3-Phosphate Synthase Functions as a Transcriptional Repressor in Populus.

Identifieur interne : 001095 ( Main/Exploration ); précédent : 001094; suivant : 001096

A 5-Enolpyruvylshikimate 3-Phosphate Synthase Functions as a Transcriptional Repressor in Populus.

Auteurs : Meng Xie [États-Unis] ; Wellington Muchero [États-Unis] ; Anthony C. Bryan [États-Unis] ; Kelsey Yee [États-Unis] ; Hao-Bo Guo [États-Unis] ; Jin Zhang [États-Unis] ; Timothy J. Tschaplinski [États-Unis] ; Vasanth R. Singan [États-Unis] ; Erika Lindquist [États-Unis] ; Raja S. Payyavula [États-Unis] ; Jaime Barros-Rios [États-Unis] ; Richard Dixon [États-Unis] ; Nancy Engle [États-Unis] ; Robert W. Sykes [États-Unis] ; Mark Davis [États-Unis] ; Sara S. Jawdy [États-Unis] ; Lee E. Gunter [États-Unis] ; Olivia Thompson [États-Unis] ; Stephen P. Difazio [États-Unis] ; Luke M. Evans [États-Unis] ; Kim Winkeler [États-Unis] ; Cassandra Collins [États-Unis] ; Jeremy Schmutz [États-Unis] ; Hong Guo [États-Unis] ; Udaya Kalluri [États-Unis] ; Miguel Rodriguez [États-Unis] ; Kai Feng [États-Unis] ; Jin-Gui Chen [États-Unis] ; Gerald A. Tuskan [États-Unis]

Source :

The Plant cell [ 1532-298X ] ; 2018.

RBID : pubmed:29891568

Descripteurs français

English descriptors

KwdEn :
- 3-Phosphoshikimate 1-Carboxyvinyltransferase (genetics), 3-Phosphoshikimate 1-Carboxyvinyltransferase (metabolism), Gene Expression Regulation, Plant (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (metabolism), Transcription Factors (genetics), Transcription Factors (metabolism).
MESH :
- chemical , genetics : 3-Phosphoshikimate 1-Carboxyvinyltransferase, Plant Proteins, Transcription Factors.
- chemical , metabolism : 3-Phosphoshikimate 1-Carboxyvinyltransferase, Plant Proteins, Transcription Factors.
- genetics : Plants, Genetically Modified, Populus.
- metabolism : Plants, Genetically Modified, Populus.
- Gene Expression Regulation, Plant.

Abstract

Long-lived perennial plants, with distinctive habits of inter-annual growth, defense, and physiology, are of great economic and ecological importance. However, some biological mechanisms resulting from genome duplication and functional divergence of genes in these systems remain poorly studied. Here, we discovered an association between a poplar (Populus trichocarpa) 5-enolpyruvylshikimate 3-phosphate synthase gene (PtrEPSP) and lignin biosynthesis. Functional characterization of PtrEPSP revealed that this isoform possesses a helix-turn-helix motif in the N terminus and can function as a transcriptional repressor that regulates expression of genes in the phenylpropanoid pathway in addition to performing its canonical biosynthesis function in the shikimate pathway. We demonstrated that this isoform can localize in the nucleus and specifically binds to the promoter and represses the expression of a SLEEPER-like transcriptional regulator, which itself specifically binds to the promoter and represses the expression of PtrMYB021 (known as MYB46 in Arabidopsis thaliana), a master regulator of the phenylpropanoid pathway and lignin biosynthesis. Analyses of overexpression and RNAi lines targeting PtrEPSP confirmed the predicted changes in PtrMYB021 expression patterns. These results demonstrate that PtrEPSP in its regulatory form and PtrhAT form a transcriptional hierarchy regulating phenylpropanoid pathway and lignin biosynthesis in Populus.

DOI: 10.1105/tpc.18.00168
PubMed: 29891568
PubMed Central: PMC6096593

Affiliations:

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Le document en format XML

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<author><name sortKey="Guo, Hong" sort="Guo, Hong" uniqKey="Guo H" first="Hong" last="Guo">Hong Guo</name>
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<country xml:lang="fr">États-Unis</country>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">A 5-Enolpyruvylshikimate 3-Phosphate Synthase Functions as a Transcriptional Repressor in <i>Populus</i>
.</title>
<author><name sortKey="Xie, Meng" sort="Xie, Meng" uniqKey="Xie M" first="Meng" last="Xie">Meng Xie</name>
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<author><name sortKey="Muchero, Wellington" sort="Muchero, Wellington" uniqKey="Muchero W" first="Wellington" last="Muchero">Wellington Muchero</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 mucherow@ornl.gov chenj@ornl.gov tuskanga@ornl.gov.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
</affiliation>
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<author><name sortKey="Bryan, Anthony C" sort="Bryan, Anthony C" uniqKey="Bryan A" first="Anthony C" last="Bryan">Anthony C. Bryan</name>
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<country xml:lang="fr">États-Unis</country>
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<author><name sortKey="Yee, Kelsey" sort="Yee, Kelsey" uniqKey="Yee K" first="Kelsey" last="Yee">Kelsey Yee</name>
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<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
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<author><name sortKey="Guo, Hao Bo" sort="Guo, Hao Bo" uniqKey="Guo H" first="Hao-Bo" last="Guo">Hao-Bo Guo</name>
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<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville</wicri:cityArea>
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<author><name sortKey="Zhang, Jin" sort="Zhang, Jin" uniqKey="Zhang J" first="Jin" last="Zhang">Jin Zhang</name>
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<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
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<author><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name>
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<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Singan, Vasanth R" sort="Singan, Vasanth R" uniqKey="Singan V" first="Vasanth R" last="Singan">Vasanth R. Singan</name>
<affiliation wicri:level="2"><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California 94598.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>U.S. Department of Energy Joint Genome Institute, Walnut Creek</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Lindquist, Erika" sort="Lindquist, Erika" uniqKey="Lindquist E" first="Erika" last="Lindquist">Erika Lindquist</name>
<affiliation wicri:level="2"><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California 94598.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>U.S. Department of Energy Joint Genome Institute, Walnut Creek</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Payyavula, Raja S" sort="Payyavula, Raja S" uniqKey="Payyavula R" first="Raja S" last="Payyavula">Raja S. Payyavula</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Barros Rios, Jaime" sort="Barros Rios, Jaime" uniqKey="Barros Rios J" first="Jaime" last="Barros-Rios">Jaime Barros-Rios</name>
<affiliation wicri:level="2"><nlm:affiliation>BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, Texas 76203.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Texas</region>
</placeName>
<wicri:cityArea>BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Dixon, Richard" sort="Dixon, Richard" uniqKey="Dixon R" first="Richard" last="Dixon">Richard Dixon</name>
<affiliation wicri:level="2"><nlm:affiliation>BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, Texas 76203.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Texas</region>
</placeName>
<wicri:cityArea>BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Engle, Nancy" sort="Engle, Nancy" uniqKey="Engle N" first="Nancy" last="Engle">Nancy Engle</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Sykes, Robert W" sort="Sykes, Robert W" uniqKey="Sykes R" first="Robert W" last="Sykes">Robert W. Sykes</name>
<affiliation wicri:level="2"><nlm:affiliation>Bioscience Center, National Renewable Energy Laboratory, Golden, Colorado 80401.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Colorado</region>
</placeName>
<wicri:cityArea>Bioscience Center, National Renewable Energy Laboratory, Golden</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Davis, Mark" sort="Davis, Mark" uniqKey="Davis M" first="Mark" last="Davis">Mark Davis</name>
<affiliation wicri:level="2"><nlm:affiliation>Bioscience Center, National Renewable Energy Laboratory, Golden, Colorado 80401.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Colorado</region>
</placeName>
<wicri:cityArea>Bioscience Center, National Renewable Energy Laboratory, Golden</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Jawdy, Sara S" sort="Jawdy, Sara S" uniqKey="Jawdy S" first="Sara S" last="Jawdy">Sara S. Jawdy</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Gunter, Lee E" sort="Gunter, Lee E" uniqKey="Gunter L" first="Lee E" last="Gunter">Lee E. Gunter</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Thompson, Olivia" sort="Thompson, Olivia" uniqKey="Thompson O" first="Olivia" last="Thompson">Olivia Thompson</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Difazio, Stephen P" sort="Difazio, Stephen P" uniqKey="Difazio S" first="Stephen P" last="Difazio">Stephen P. Difazio</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biology, West Virginia University, Morgantown, West Virginia 26506.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Virginie-Occidentale</region>
</placeName>
<wicri:cityArea>Department of Biology, West Virginia University, Morgantown</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Evans, Luke M" sort="Evans, Luke M" uniqKey="Evans L" first="Luke M" last="Evans">Luke M. Evans</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biology, West Virginia University, Morgantown, West Virginia 26506.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Virginie-Occidentale</region>
</placeName>
<wicri:cityArea>Department of Biology, West Virginia University, Morgantown</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Winkeler, Kim" sort="Winkeler, Kim" uniqKey="Winkeler K" first="Kim" last="Winkeler">Kim Winkeler</name>
<affiliation wicri:level="2"><nlm:affiliation>ArborGen, Ridgeville, South Carolina 29472.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Caroline du Sud</region>
</placeName>
<wicri:cityArea>ArborGen, Ridgeville</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Collins, Cassandra" sort="Collins, Cassandra" uniqKey="Collins C" first="Cassandra" last="Collins">Cassandra Collins</name>
<affiliation wicri:level="2"><nlm:affiliation>ArborGen, Ridgeville, South Carolina 29472.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Caroline du Sud</region>
</placeName>
<wicri:cityArea>ArborGen, Ridgeville</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Schmutz, Jeremy" sort="Schmutz, Jeremy" uniqKey="Schmutz J" first="Jeremy" last="Schmutz">Jeremy Schmutz</name>
<affiliation wicri:level="2"><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California 94598.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>U.S. Department of Energy Joint Genome Institute, Walnut Creek</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><nlm:affiliation>HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Alabama</region>
</placeName>
<wicri:cityArea>HudsonAlpha Institute for Biotechnology, Huntsville</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Guo, Hong" sort="Guo, Hong" uniqKey="Guo H" first="Hong" last="Guo">Hong Guo</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Kalluri, Udaya" sort="Kalluri, Udaya" uniqKey="Kalluri U" first="Udaya" last="Kalluri">Udaya Kalluri</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Rodriguez, Miguel" sort="Rodriguez, Miguel" uniqKey="Rodriguez M" first="Miguel" last="Rodriguez">Miguel Rodriguez</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Feng, Kai" sort="Feng, Kai" uniqKey="Feng K" first="Kai" last="Feng">Kai Feng</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Chen, Jin Gui" sort="Chen, Jin Gui" uniqKey="Chen J" first="Jin-Gui" last="Chen">Jin-Gui Chen</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 mucherow@ornl.gov chenj@ornl.gov tuskanga@ornl.gov.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name>
<affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 mucherow@ornl.gov chenj@ornl.gov tuskanga@ornl.gov.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Tennessee</region>
</placeName>
<wicri:cityArea>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California 94598.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>U.S. Department of Energy Joint Genome Institute, Walnut Creek</wicri:cityArea>
</affiliation>
</author>
</analytic>
<series><title level="j">The Plant cell</title>
<idno type="eISSN">1532-298X</idno>
<imprint><date when="2018" type="published">2018</date>
</imprint>
</series>
</biblStruct>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3-Phosphoshikimate 1-Carboxyvinyltransferase (genetics)</term>
<term>3-Phosphoshikimate 1-Carboxyvinyltransferase (metabolism)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (metabolism)</term>
<term>Populus (genetics)</term>
<term>Populus (metabolism)</term>
<term>Transcription Factors (genetics)</term>
<term>Transcription Factors (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>3-Phosphoshikimate 1-carboxyvinyltransferase (génétique)</term>
<term>3-Phosphoshikimate 1-carboxyvinyltransferase (métabolisme)</term>
<term>Facteurs de transcription (génétique)</term>
<term>Facteurs de transcription (métabolisme)</term>
<term>Populus (génétique)</term>
<term>Populus (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
<term>Végétaux génétiquement modifiés (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>3-Phosphoshikimate 1-Carboxyvinyltransferase</term>
<term>Plant Proteins</term>
<term>Transcription Factors</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>3-Phosphoshikimate 1-Carboxyvinyltransferase</term>
<term>Plant Proteins</term>
<term>Transcription Factors</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>3-Phosphoshikimate 1-carboxyvinyltransferase</term>
<term>Facteurs de transcription</term>
<term>Populus</term>
<term>Protéines végétales</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>3-Phosphoshikimate 1-carboxyvinyltransferase</term>
<term>Facteurs de transcription</term>
<term>Populus</term>
<term>Protéines végétales</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Régulation de l'expression des gènes végétaux</term>
</keywords>
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<front><div type="abstract" xml:lang="en">Long-lived perennial plants, with distinctive habits of inter-annual growth, defense, and physiology, are of great economic and ecological importance. However, some biological mechanisms resulting from genome duplication and functional divergence of genes in these systems remain poorly studied. Here, we discovered an association between a poplar (<i>Populus trichocarpa)</i>
 5-enolpyruvylshikimate 3-phosphate synthase gene (<i>PtrEPSP</i>
) and lignin biosynthesis. Functional characterization of PtrEPSP revealed that this isoform possesses a helix-turn-helix motif in the N terminus and can function as a transcriptional repressor that regulates expression of genes in the phenylpropanoid pathway in addition to performing its canonical biosynthesis function in the shikimate pathway. We demonstrated that this isoform can localize in the nucleus and specifically binds to the promoter and represses the expression of a <i>SLEEPER</i>
-like transcriptional regulator, which itself specifically binds to the promoter and represses the expression of <i>PtrMYB021</i>
 (known as <i>MYB46</i>
 in <i>Arabidopsis thaliana</i>
), a master regulator of the phenylpropanoid pathway and lignin biosynthesis. Analyses of overexpression and RNAi lines targeting PtrEPSP confirmed the predicted changes in <i>PtrMYB021</i>
 expression patterns. These results demonstrate that PtrEPSP in its regulatory form and PtrhAT form a transcriptional hierarchy regulating phenylpropanoid pathway and lignin biosynthesis in <i>Populus</i>
.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29891568</PMID>
<DateCompleted><Year>2019</Year>
<Month>06</Month>
<Day>17</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>06</Month>
<Day>17</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1532-298X</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>30</Volume>
<Issue>7</Issue>
<PubDate><Year>2018</Year>
<Month>07</Month>
</PubDate>
</JournalIssue>
<Title>The Plant cell</Title>
<ISOAbbreviation>Plant Cell</ISOAbbreviation>
</Journal>
<ArticleTitle>A 5-Enolpyruvylshikimate 3-Phosphate Synthase Functions as a Transcriptional Repressor in <i>Populus</i>
.</ArticleTitle>
<Pagination><MedlinePgn>1645-1660</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1105/tpc.18.00168</ELocationID>
<Abstract><AbstractText>Long-lived perennial plants, with distinctive habits of inter-annual growth, defense, and physiology, are of great economic and ecological importance. However, some biological mechanisms resulting from genome duplication and functional divergence of genes in these systems remain poorly studied. Here, we discovered an association between a poplar (<i>Populus trichocarpa)</i>
 5-enolpyruvylshikimate 3-phosphate synthase gene (<i>PtrEPSP</i>
) and lignin biosynthesis. Functional characterization of PtrEPSP revealed that this isoform possesses a helix-turn-helix motif in the N terminus and can function as a transcriptional repressor that regulates expression of genes in the phenylpropanoid pathway in addition to performing its canonical biosynthesis function in the shikimate pathway. We demonstrated that this isoform can localize in the nucleus and specifically binds to the promoter and represses the expression of a <i>SLEEPER</i>
-like transcriptional regulator, which itself specifically binds to the promoter and represses the expression of <i>PtrMYB021</i>
 (known as <i>MYB46</i>
 in <i>Arabidopsis thaliana</i>
), a master regulator of the phenylpropanoid pathway and lignin biosynthesis. Analyses of overexpression and RNAi lines targeting PtrEPSP confirmed the predicted changes in <i>PtrMYB021</i>
 expression patterns. These results demonstrate that PtrEPSP in its regulatory form and PtrhAT form a transcriptional hierarchy regulating phenylpropanoid pathway and lignin biosynthesis in <i>Populus</i>
.</AbstractText>
<CopyrightInformation>© 2018 American Society of Plant Biologists. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xie</LastName>
<ForeName>Meng</ForeName>
<Initials>M</Initials>
<Identifier Source="ORCID">0000-0003-0247-3701</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Muchero</LastName>
<ForeName>Wellington</ForeName>
<Initials>W</Initials>
<Identifier Source="ORCID">0000-0002-0200-9856</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 mucherow@ornl.gov chenj@ornl.gov tuskanga@ornl.gov.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bryan</LastName>
<ForeName>Anthony C</ForeName>
<Initials>AC</Initials>
<Identifier Source="ORCID">0000-0003-0917-8705</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yee</LastName>
<ForeName>Kelsey</ForeName>
<Initials>K</Initials>
<Identifier Source="ORCID">0000-0002-7219-0638</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Guo</LastName>
<ForeName>Hao-Bo</ForeName>
<Initials>HB</Initials>
<Identifier Source="ORCID">0000-0003-1321-1758</Identifier>
<AffiliationInfo><Affiliation>Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Jin</ForeName>
<Initials>J</Initials>
<Identifier Source="ORCID">0000-0002-8397-5078</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tschaplinski</LastName>
<ForeName>Timothy J</ForeName>
<Initials>TJ</Initials>
<Identifier Source="ORCID">0000-0002-9540-6622</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Singan</LastName>
<ForeName>Vasanth R</ForeName>
<Initials>VR</Initials>
<Identifier Source="ORCID">0000-0002-9983-5707</Identifier>
<AffiliationInfo><Affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California 94598.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lindquist</LastName>
<ForeName>Erika</ForeName>
<Initials>E</Initials>
<Identifier Source="ORCID">0000-0003-2249-7225</Identifier>
<AffiliationInfo><Affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California 94598.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Payyavula</LastName>
<ForeName>Raja S</ForeName>
<Initials>RS</Initials>
<Identifier Source="ORCID">0000-0001-8945-5041</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Barros-Rios</LastName>
<ForeName>Jaime</ForeName>
<Initials>J</Initials>
<Identifier Source="ORCID">0000-0002-9545-312X</Identifier>
<AffiliationInfo><Affiliation>BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, Texas 76203.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Dixon</LastName>
<ForeName>Richard</ForeName>
<Initials>R</Initials>
<Identifier Source="ORCID">0000-0001-8393-9408</Identifier>
<AffiliationInfo><Affiliation>BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, Texas 76203.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Engle</LastName>
<ForeName>Nancy</ForeName>
<Initials>N</Initials>
<Identifier Source="ORCID">0000-0003-0290-7987</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sykes</LastName>
<ForeName>Robert W</ForeName>
<Initials>RW</Initials>
<Identifier Source="ORCID">0000-0002-2061-6625</Identifier>
<AffiliationInfo><Affiliation>Bioscience Center, National Renewable Energy Laboratory, Golden, Colorado 80401.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Davis</LastName>
<ForeName>Mark</ForeName>
<Initials>M</Initials>
<Identifier Source="ORCID">0000-0003-4541-9852</Identifier>
<AffiliationInfo><Affiliation>Bioscience Center, National Renewable Energy Laboratory, Golden, Colorado 80401.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jawdy</LastName>
<ForeName>Sara S</ForeName>
<Initials>SS</Initials>
<Identifier Source="ORCID">0000-0002-8123-5439</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation>
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</Author>
<Author ValidYN="Y"><LastName>Gunter</LastName>
<ForeName>Lee E</ForeName>
<Initials>LE</Initials>
<Identifier Source="ORCID">0000-0003-1211-7532</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Thompson</LastName>
<ForeName>Olivia</ForeName>
<Initials>O</Initials>
<Identifier Source="ORCID">0000-0003-2969-4774</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>DiFazio</LastName>
<ForeName>Stephen P</ForeName>
<Initials>SP</Initials>
<Identifier Source="ORCID">0000-0003-4077-1590</Identifier>
<AffiliationInfo><Affiliation>Department of Biology, West Virginia University, Morgantown, West Virginia 26506.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Evans</LastName>
<ForeName>Luke M</ForeName>
<Initials>LM</Initials>
<Identifier Source="ORCID">0000-0002-7458-1720</Identifier>
<AffiliationInfo><Affiliation>Department of Biology, West Virginia University, Morgantown, West Virginia 26506.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Winkeler</LastName>
<ForeName>Kim</ForeName>
<Initials>K</Initials>
<Identifier Source="ORCID">0000-0002-7634-5422</Identifier>
<AffiliationInfo><Affiliation>ArborGen, Ridgeville, South Carolina 29472.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Collins</LastName>
<ForeName>Cassandra</ForeName>
<Initials>C</Initials>
<Identifier Source="ORCID">0000-0001-9763-2132</Identifier>
<AffiliationInfo><Affiliation>ArborGen, Ridgeville, South Carolina 29472.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Schmutz</LastName>
<ForeName>Jeremy</ForeName>
<Initials>J</Initials>
<Identifier Source="ORCID">0000-0001-8062-9172</Identifier>
<AffiliationInfo><Affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California 94598.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Guo</LastName>
<ForeName>Hong</ForeName>
<Initials>H</Initials>
<Identifier Source="ORCID">0000-0002-5997-5086</Identifier>
<AffiliationInfo><Affiliation>Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kalluri</LastName>
<ForeName>Udaya</ForeName>
<Initials>U</Initials>
<Identifier Source="ORCID">0000-0002-5963-8370</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Rodriguez</LastName>
<ForeName>Miguel</ForeName>
<Initials>M</Initials>
<Identifier Source="ORCID">0000-0001-5890-051X</Identifier>
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</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Feng</LastName>
<ForeName>Kai</ForeName>
<Initials>K</Initials>
<Identifier Source="ORCID">0000-0002-9903-6497</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Jin-Gui</ForeName>
<Initials>JG</Initials>
<Identifier Source="ORCID">0000-0002-1752-4201</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 mucherow@ornl.gov chenj@ornl.gov tuskanga@ornl.gov.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tuskan</LastName>
<ForeName>Gerald A</ForeName>
<Initials>GA</Initials>
<Identifier Source="ORCID">0000-0003-0106-1289</Identifier>
<AffiliationInfo><Affiliation>BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 mucherow@ornl.gov chenj@ornl.gov tuskanga@ornl.gov.</Affiliation>
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<ArticleId IdType="pii">tpc.18.00168</ArticleId>
<ArticleId IdType="doi">10.1105/tpc.18.00168</ArticleId>
<ArticleId IdType="pmc">PMC6096593</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Chem Theory Comput. 2012 Sep 11;8(9):3257-3273</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23341755</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Plant Biol. 2012;63:73-105</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22554242</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013 Jul 29;8(7):e69219</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23922694</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2007;2(7):1565-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17585298</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Genet. 2008 May;9(5):397-405</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18368054</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEMS Microbiol Rev. 2005 Apr;29(2):231-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15808743</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20057044</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Plant Biol. 2012 Oct 16;12:192</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23067104</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2013 Jan;73(1):26-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26011122</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant. 2010 Jan;3(1):2-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20035037</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Comput Chem. 2009 Jul 30;30(10):1545-614</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19444816</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Biofuels. 2012 Sep 21;5(1):71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22998926</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2005 Aug;43(4):553-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16098109</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2013 Apr 25;14(4):R36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23618408</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2003 Aug 12;19(12):1572-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12912839</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Proteome Res. 2015 Oct 2;14(10):4158-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26325666</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Crit Rev Microbiol. 2015 Jun;41(2):172-89</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23919299</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2007 Jan;19(1):270-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17237351</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2014 Feb;164(2):765-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24394777</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Biofuels. 2012 Jan 04;5(1):2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22214220</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2011 Aug;67(3):499-512</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21649762</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Evol. 2003 Sep;20(9):1377-419</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12777501</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteins. 2014 Feb;82 Suppl 2:175-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23760925</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Bioinformatics. 2006 Aug 16;7:382</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16914055</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Proteomics. 2015 Aug 3;126:94-108</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26047713</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2006 Jan 1;34(Database issue):D247-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16381856</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2012 Jul;40(Web Server issue):W471-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22570420</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 2014 Aug;85(6):589-99</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24879533</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2004 Mar 19;32(5):1792-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15034147</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Plant Sci. 2013 Mar 27;4:62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23543266</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2011 May;66(4):579-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21284754</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Comput Chem. 2005 Dec;26(16):1781-802</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16222654</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2010 Apr;5(4):725-38</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20360767</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2006 Jan 27;311(5760):484-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16439654</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Physiol. 2010 Jun;51(6):1084-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20427511</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2014 Jul;203(2):535-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24750093</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2014 Sep;9(9):2180-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25144269</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2011 Sep 29;6(10):1656-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21959244</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Plant Sci. 2013 Aug 29;4:325</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24009617</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Biotechnol. 2008 Apr;19(2):166-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18403196</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Res. 2014 Oct;24(10):1274-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24980958</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Plant Biol. 2014 Oct 07;14:265</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25287590</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2006 Sep 15;313(5793):1596-604</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16973872</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Graph. 1996 Feb;14(1):33-8, 27-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8744570</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Comput Biol. 2011 Oct;7(10):e1002195</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22039361</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Pac Symp Biocomput. 2005;:151-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15759622</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2005 Jul 14;436(7048):282-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16015335</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Genomics. 2015 Jan 23;16:24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25613058</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2010 Oct;22(10):3461-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20952636</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Genet. 2006 Feb;38(2):203-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16380716</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 1994 Dec;14(2-3):94-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24192872</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2014 Apr 1;30(7):923-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24227677</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10371-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24958886</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2003 Feb;15(2):533-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12566590</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2012;7(9):e44908</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23028673</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arabidopsis Book. 2011;9:e0152</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22303276</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>In Vitro Cell Dev Biol Plant. 2009 Dec;45(6):619-629</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19936031</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2009 Feb;149(2):981-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19091872</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2008 Oct;20(10):2763-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18952777</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biochem Biophys Methods. 1982 Dec;7(1):7-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7153458</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Tree Physiol. 2009 Mar;29(3):333-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19203957</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2010 Nov 22;5(11):e14021</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21151641</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2007 Sep;19(9):2776-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17890373</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2012 May;40(10):4422-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22302148</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2016 Jul 26;113(30):E4415-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27407149</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Res Notes. 2014 Jan 22;7:58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24450620</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Ann Bot. 2015 Jun;115(7):1053-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25878140</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2003 Dec;36(6):743-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14675440</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2012 Nov;196(3):726-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22943289</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2008;3(6):1032-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18536650</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Ecol Resour. 2013 Mar;13(2):306-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23311503</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Methods. 2008 Oct 15;4:24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18922163</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Signal Behav. 2010 Apr;5(4):469-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20383071</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2010 Jul;153(3):895-905</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20472751</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2014;15(12):550</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25516281</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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<name sortKey="Davis, Mark" sort="Davis, Mark" uniqKey="Davis M" first="Mark" last="Davis">Mark Davis</name>
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<name sortKey="Engle, Nancy" sort="Engle, Nancy" uniqKey="Engle N" first="Nancy" last="Engle">Nancy Engle</name>
<name sortKey="Evans, Luke M" sort="Evans, Luke M" uniqKey="Evans L" first="Luke M" last="Evans">Luke M. Evans</name>
<name sortKey="Feng, Kai" sort="Feng, Kai" uniqKey="Feng K" first="Kai" last="Feng">Kai Feng</name>
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<name sortKey="Guo, Hong" sort="Guo, Hong" uniqKey="Guo H" first="Hong" last="Guo">Hong Guo</name>
<name sortKey="Jawdy, Sara S" sort="Jawdy, Sara S" uniqKey="Jawdy S" first="Sara S" last="Jawdy">Sara S. Jawdy</name>
<name sortKey="Kalluri, Udaya" sort="Kalluri, Udaya" uniqKey="Kalluri U" first="Udaya" last="Kalluri">Udaya Kalluri</name>
<name sortKey="Lindquist, Erika" sort="Lindquist, Erika" uniqKey="Lindquist E" first="Erika" last="Lindquist">Erika Lindquist</name>
<name sortKey="Muchero, Wellington" sort="Muchero, Wellington" uniqKey="Muchero W" first="Wellington" last="Muchero">Wellington Muchero</name>
<name sortKey="Payyavula, Raja S" sort="Payyavula, Raja S" uniqKey="Payyavula R" first="Raja S" last="Payyavula">Raja S. Payyavula</name>
<name sortKey="Rodriguez, Miguel" sort="Rodriguez, Miguel" uniqKey="Rodriguez M" first="Miguel" last="Rodriguez">Miguel Rodriguez</name>
<name sortKey="Schmutz, Jeremy" sort="Schmutz, Jeremy" uniqKey="Schmutz J" first="Jeremy" last="Schmutz">Jeremy Schmutz</name>
<name sortKey="Schmutz, Jeremy" sort="Schmutz, Jeremy" uniqKey="Schmutz J" first="Jeremy" last="Schmutz">Jeremy Schmutz</name>
<name sortKey="Singan, Vasanth R" sort="Singan, Vasanth R" uniqKey="Singan V" first="Vasanth R" last="Singan">Vasanth R. Singan</name>
<name sortKey="Sykes, Robert W" sort="Sykes, Robert W" uniqKey="Sykes R" first="Robert W" last="Sykes">Robert W. Sykes</name>
<name sortKey="Thompson, Olivia" sort="Thompson, Olivia" uniqKey="Thompson O" first="Olivia" last="Thompson">Olivia Thompson</name>
<name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name>
<name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name>
<name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name>
<name sortKey="Winkeler, Kim" sort="Winkeler, Kim" uniqKey="Winkeler K" first="Kim" last="Winkeler">Kim Winkeler</name>
<name sortKey="Yee, Kelsey" sort="Yee, Kelsey" uniqKey="Yee K" first="Kelsey" last="Yee">Kelsey Yee</name>
<name sortKey="Zhang, Jin" sort="Zhang, Jin" uniqKey="Zhang J" first="Jin" last="Zhang">Jin Zhang</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001095 | SxmlIndent | more

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{{Explor lien
   |wiki=    Bois
   |area=    PoplarV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:29891568
   |texte=   A 5-Enolpyruvylshikimate 3-Phosphate Synthase Functions as a Transcriptional Repressor in Populus.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:29891568" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PoplarV1

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A 5-Enolpyruvylshikimate 3-Phosphate Synthase Functions as a Transcriptional Repressor in Populus.

A 5-Enolpyruvylshikimate 3-Phosphate Synthase Functions as a Transcriptional Repressor in Populus.

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Abstract

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